F1112703

Anti-Human CD127 (IL-7Rα), APC (Clone: A019D5)流式抗体 (新品)

¥1,112.00 – ¥2,464.00

说明书

分子靶点：CD127, IL7R, IL7RA
种属：人 (Human)
标记：APC
亚型：Mouse IgG1, κ
克隆号：A019D5

在售SKU, 70-AH012705-20, 70-AH012705-50, 70-AH012705-100, AH012705, 70-F1112703-25, 70-F1112703-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
IL7R 分子靶点信息概述
IL7R 分子靶点综述
- 人 Human IL7R 分子靶点信息
- 人 Human IL7R靶点分子功能（预测）

商品名	Anti-Human CD127 (IL-7Rα), APC (Clone:RDR5)流式抗体
靶点	CD127, IL7R, IL7RA
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	A019D5
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD127 (IL-7Rα), APC (F1112703) 和 Anti-Human CD3, FITC(F11003A01) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD127, IL7R, IL7RA 靶点信息

IL7R 分子靶点信息概述

分子名：IL7R, interleukin 7 receptor
基因家族：CD molecules; Fibronectin type III domain containing; Interleukin receptors
别名：CD127; IL7RA

IL7R 分子靶点综述

CD127是白细胞介素7受体α链，与CD132 (β链)共同形成高亲和力的受体。CD127表达于大部分T细胞，但在T细胞活化后可发生降低，也表达在B细胞前体和单核细胞。CD127的表达对T淋巴细胞的成熟、维持及免疫应答发挥着重要作用。

人 Human IL7R 分子靶点信息

分子名：IL7R, interleukin 7 receptor
别称：
- CD127
- CD127 antigen
- CDW127
- IL-7 receptor subunit alpha
- IL-7R subunit alpha
- IL-7R-alpha
- IL-7RA
- IL7RA
- ILRA
- interleukin 7 receptor alpha chain
- interleukin 7 receptor isoform H5-6
- interleukin-7 receptor subunit alpha
基因序列：NCBI_Gene: 3575
蛋白序列：UniProtKB: P16871

人 Human IL7R靶点分子功能（预测）

Enables cytokine receptor activity. Involved in positive regulation of cell population proliferation and positive regulation of receptor signaling pathway via STAT. Located in cytosol; nucleoplasm; and plasma membrane. Implicated in severe combined immunodeficiency and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive.

操作步骤

文章目录[隐藏]

结果示例
分子信息
IL7R 分子靶点信息概述
IL7R 分子靶点综述
- 人 Human IL7R 分子靶点信息
- 人 Human IL7R靶点分子功能（预测）
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 50 - 100 μl 抗凝血，加入 5 μl Anti-Human CD127 (IL-7Rα), APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD127 (IL-7Rα), APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5- 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。