F1122301

Anti-Human CD223, FITC （Clone: OTI5D8）检测试剂

¥1,360.00 – ¥3,368.00

说明书

分子靶点：CD223, LAG3
种属：人 (Human)
标记：FITC
亚型：Mouse IgG1
克隆号：OTI5D8

在售SKU, F1122301, 70-F1122301-100, 70-F1122301-25

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
LAG3 分子靶点信息概述
LAG3 分子靶点综述
- 人 Human LAG3 分子靶点信息
- 人 Human LAG3靶点分子功能（预测）

商品名	Anti-Human CD223, FITC （Clone: OTI5D8）检测试剂
靶点	CD223, LAG3
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Mouse IgG1
克隆号	OTI5D8
推荐应用	流式检测
用量	5 μL/Test
纯化	亲和层析
储存液	含 0.2% BSA 和0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - CD3、CD28抗体诱导的人PBMC染色左图为Mouse IgG1 Isotype control, FITC和Anti-Human CD4, APC的结果，右图为Anti-Human CD223, FITC(F1122301)和Anti-Human CD4, APC的结果，以全部细胞设门。

分子信息

概述人 CD223, LAG3 靶点信息

LAG3 分子靶点信息概述

分子名：LAG3, lymphocyte activating 3
基因家族：CD molecules; Immunoglobulin like domain containing
别名：CD223
全称：lymphocyte-activation gene 3

LAG3 分子靶点综述

CD223，即淋巴细胞活化基因3（LAG-3），其结合MHC-II比CD4具有更高亲和力，所以被认为与T细胞活化的负调控以及稳态增殖有关。LAG-3有报道说在活化的T细胞（包括调节性T细胞）以及NK细胞上表达。CD8+T细胞上通常比CD4+T细胞表达更多的LAG-3。 LAG-3与CD49b的共表达被推荐用于鉴定人和小鼠的Tr1细胞。

人 Human LAG3 分子靶点信息

分子名：LAG3, lymphocyte activating 3
别称：
- CD223
- lymphocyte activation gene 3 protein
- lymphocyte-activation gene 3
基因序列：NCBI_Gene: 3902
蛋白序列：UniProtKB: P18627

人 Human LAG3靶点分子功能（预测）

Enables MHC class II protein binding activity. Predicted to be involved in several processes, including cell surface receptor signaling pathway; negative regulation of regulatory T cell differentiation; and plasmacytoid dendritic cell activation. Predicted to act upstream of or within negative regulation of T cell activation; negative regulation of interleukin-2 production; and positive regulation of natural killer cell mediated cytotoxicity. Predicted to be located in extracellular region.

操作步骤

文章目录[隐藏]

结果示例
分子信息
LAG3 分子靶点信息概述
LAG3 分子靶点综述
- 人 Human LAG3 分子靶点信息
- 人 Human LAG3靶点分子功能（预测）
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取100 μl 抗凝血，加入5 μl Anti-Human CD223, FITC 和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl
Anti-Human CD223, FITC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵
育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 -
10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。