F1100305

Anti-Human CD3，PE-Cy7 人 CD3 流式抗体 (新品)

¥1,152.00 – ¥2,560.00

说明书

分子靶点：CD3
种属：人 (Human)
标记：PE-Cy7
亚型：Mouse IgG2a, κ
克隆号：OKT3

在售SKU:70-F1100305-25, 70-F1100305-100, 人, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3

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Size
库存号

描述

商品名	Anti-Human CD3, PE-Cy7 (Clone: OKT3) 流式抗体
靶点	CD3
标记	PE-Cy7
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG2a, κ
克隆号	OKT3
推荐应用	流式检测
用量	5 μL (0.25 μg)/Test
纯化	亲和层析
储存液	含0.2% BSA和0.02% ProClin950的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取100 μl抗凝血，加入5 μl Anti-Human CD3, PE-Cy7和其他抗体，震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g离心5 - 10分钟，弃上清，加入500 μl流式染色缓冲液重悬，上机检测。
其他样本：将1×10⁵ - 10⁸个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD3, PE-Cy7和其他抗体，使反应终体积为100 μl。震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入1 - 2 ml流式染色缓冲液，300 × g离心5 - 10分钟，弃上清，加入500 μl流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。