F1136601

Anti-Human CD366, FITC （Clone: 1E3）检测试剂

¥1,160.00 – ¥2,960.00

说明书

分子靶点：CD366, TIM3, HAVCR2
种属：人 (Human)
标记：FITC
亚型：Rat IgG1
克隆号：1E3

在售SKU, F1136601, 70-F1136601-25, 70-F1136601-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
HAVCR2 分子靶点信息概述
HAVCR2 分子靶点综述
- 人 Human HAVCR2 分子靶点信息
- 人 Human HAVCR2靶点分子功能（预测）

商品名	Anti-Human CD366, FITC （Clone: 1E3）检测试剂
靶点	CD366, TIM3, HAVCR2
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Rat IgG1
克隆号	1E3
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - CD3、CD28抗体诱导的人PBMC染色左图为Mouse IgG1 Isotype control, FITC和Anti-Human CD4, APC的结果，右图为Anti-Human CD366, FITC(F1136601)和Anti-Human CD4, APC的结果，以全部细胞设门。

分子信息

概述人 CD366, TIM3, HAVCR2 靶点信息

HAVCR2 分子靶点信息概述

分子名：HAVCR2, hepatitis A virus cellular receptor 2
基因家族：V-set domain containing
别名：Tim-3; TIM3; FLJ14428; TIMD3; CD366
全称：T-cell immunoglobulin mucin family member 3

HAVCR2 分子靶点综述

TIM3（甲型肝炎病毒细胞受体2，HAVCR2，T细胞免疫球蛋白）由281个氨基酸组成，类型为1的Th1特异性细胞表面糖蛋白，在终末分化的CD4 + Th1和CD8 + Tc1上表达细胞。TIM3由IgV样结构域，胞外区域的粘蛋白样结构域和胞质区域的保守的酪氨酸磷酸化基序组成。TIM3参与巨噬细胞的活化和自身免疫性疾病的发生。此外，TIM3介导的免疫反应，并促进免疫耐受。

人 Human HAVCR2 分子靶点信息

分子名：HAVCR2, hepatitis A virus cellular receptor 2
别称：
- CD366
- FLJ14428
- HAVcr-2
- kidney injury molecule-3
- KIM-3
- SPTCL
- T cell immunoglobulin mucin 3
- T cell immunoglobulin mucin-3
- T-cell immunoglobulin and mucin domain-containing protein 3
- T-cell immunoglobulin mucin family member 3
- T-cell immunoglobulin mucin receptor 3
- T-cell membrane protein 3
- Tim-3
- TIM3
- TIMD-3
- TIMD3
基因序列：NCBI_Gene: 84868
蛋白序列：UniProtKB: Q8TDQ0

人 Human HAVCR2靶点分子功能（预测）

Predicted to enable metal ion binding activity. Involved in several processes, including natural killer cell tolerance induction; negative regulation of leukocyte activation; and regulation of cytokine production. Located in cell surface. Implicated in hepatocellular carcinoma and renal cell carcinoma. Biomarker of cystic fibrosis and pulmonary tuberculosis.

操作步骤

文章目录[隐藏]

结果示例
分子信息
HAVCR2 分子靶点信息概述
HAVCR2 分子靶点综述
- 人 Human HAVCR2 分子靶点信息
- 人 Human HAVCR2靶点分子功能（预测）
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取100 μl 抗凝血，加入5 μl Anti-Human CD366, FITC 和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD366, FITC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。