F11045RO03

Anti-HumanCD45RO,APC(Clone:UCHL1)检测试剂

¥600.00 – ¥1,320.00

说明书

分子靶点：CD45, GP180, PTPRC
种属：人 (Human)
标记：APC
亚型：Mouse IgG2a, κ
克隆号：UCHL1

在售SKU, 70-AH045RO05-20, 70-AH045RO05-50, 70-AH045RO05-100, AH045RO05, 70-F11045RO03-25, 70-F11045RO03-100，70-F11045RO03/2-25, 70-F11045RO03/2-100

¥<\/span>1,320.00<\/bdi><\/span><\/span>","sku":"70-F11045RO03\/2-100","variation_description":"","variation_id":16506,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":600,"display_regular_price":600,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>600.00<\/bdi><\/span><\/span>","sku":"70-F11045RO03\/2-25","variation_description":"","variation_id":16507,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

结果示例

商品名	Anti-HumanCD45RO,APC(Clone:UCHL1)检测试剂
靶点	CD45, GP180, PTPRC
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	MouseIgG1,κ
克隆号	UCHL1
推荐应用	流式检测
用量	5μl/tes
纯化	亲和层析
储存液	含0.2%BSA和0.2%proclin950的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色Anti-HumanCD45RO,APC(F11045RO03/2)的结果。以FSC/SSC中的淋巴细胞设门进行分析。

操作步骤

文章目录[隐藏]

结果示例
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD45RO, APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 ×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD45RO, APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。