F1105901

Anti-Human CD59, FITC (Clone:FOV9A2) (新品)

¥990.00 – ¥1,760.00

说明书

分子靶点：CD59
种属：人 (Human)
标记：FITC
亚型：Mouse IgG1, κ
克隆号：FOV9A2

在售SKU, 70-AH05901-20, 70-AH05901-50, 70-AH05901-100, AH05901, 70-F1105901-25, 70-F1105901-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD59 分子靶点信息概述
CD59 分子靶点综述
- 人 Human CD59 分子靶点信息
- 人 Human CD59靶点分子功能（预测）

商品名	Anti-Human CD59, FITC (Clone:FOV9A2)
靶点	CD59
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	FOV9A2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD59, FITC(F1105901)(实线) 和 Mouse IgG1 κ Isotype Control, FITC(CMG101)(虚线)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD5, LEU1 靶点信息

CD59 分子靶点信息概述

分子名：CD59, CD59 molecule (CD59 blood group)
基因家族：Blood group antigens; CD molecules; LY6/PLAUR domain containing; Complement system regulators and receptors
别名：16.3A5; EJ16; EJ30; EL32; G344; p18-20
曾用名：MIC11; MIN1; MSK21; MIN2; MIN3
全称：CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 antigen, complement regulatory protein; CD59 molecule, complement regulatory protein; CD59 molecule

CD59 分子靶点综述

CD59 也被称为保护素，在所有人类淋巴细胞、单核细胞、粒细胞和红细胞上均有表达。这种蛋白也在非造血细胞上表达，包括内皮细胞和神经元。CD59 与 CD2 相互作用，调节 T 细胞粘附，并在 T 细胞活化中发挥作用。最后，在系统性红斑狼疮(SLE)患者中观察到 CD55和 CD59 在人外周血细胞上的表达改变。

人 Human CD59 分子靶点信息

分子名：CD59, CD59 molecule (CD59 blood group)
别称：
- 16.3A5
- 1F5
- 1F5 antigen
- 20 kDa homologous restriction factor
- cd59 antigen
- CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)
- CD59 blood group antigen
- CD59 glycoprotein
- CD59 molecule
- EJ16
- EJ30
- EL32
- FLJ38134
- FLJ92039
- G344
- HRF-20
- HRF20
- human leukocyte antigen MIC11
- Ly-6-like protein
- lymphocytic antigen CD59/MEM43
- MAC-IP
- MACIF
- MEM43
- MEM43 antigen
- membrane attack complex (MAC) inhibition factor
- membrane attack complex inhibition factor
- membrane inhibitor of reactive lysis
- MGC2354
- MIC11
- MIN1
- MIN2
- MIN3
- MIRL
- MSK21
- p18-20
- protectin
- surface anitgen recognized by monoclonal antibody 16.3A5
- T cell-activating protein
基因序列：NCBI_Gene: 966
蛋白序列：UniProtKB: P13987

人 Human CD59靶点分子功能（预测）

Predicted to enable complement binding activity. Acts upstream of or within regulation of complement activation and regulation of complement-dependent cytotoxicity. Located in cell surface. Is anchored component of external side of plasma membrane. Implicated in colorectal carcinoma and ovarian cancer. Biomarker of breast carcinoma; endometrial cancer; and ovarian cancer.

操作步骤

文章目录[隐藏]

结果示例
分子信息
CD59 分子靶点信息概述
CD59 分子靶点综述
- 人 Human CD59 分子靶点信息
- 人 Human CD59靶点分子功能（预测）
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD59, FITC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD59, FITC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。