F11045R03

Anti-Human/Mouse CD45R (B220), APC (Clone:RA3-6B2) 流式抗体 (新品)

¥208.00 – ¥440.00

说明书

分子靶点：CD45, GP180, PTPRC
种属：人 (Human); 小鼠 (Mouse)
标记：APC
亚型：Rat IgG2a, κ
克隆号：RA3-6B2

在售SKU, 70-AH045R05-20, 70-AH045R05-50, 70-AH045R05-100, AH045R05, 70-F11045R03-25, 70-F11045R03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例

商品名	Anti-Human/Mouse CD45R (B220), APC (Clone:RA3-6B2) 流式抗体
靶点	CD45, GP180, PTPRC
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Rat IgG2a, κ
克隆号	RA3-6B2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 小鼠骨髓细胞染色 Anti-Human/Mouse CD45R (B220), APC (F11045R03) 和 Anti-Human/Mouse CD11b, FITC (F11011B01) 的结果。已排除黏连细胞。

操作步骤

文章目录[隐藏]

结果示例
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 50 - 100 μl 抗凝血，加入 5 μl Anti-Human/Mouse CD45R (B220), APC 和其他抗体，震荡混于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。匀，根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human/Mouse CD45R (B220), APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 ×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。