F2112702

Anti-Mouse CD127 (IL-7Rα), PE (Clone:A7R34) 流式抗体 (新品)

¥792.00 – ¥1,760.00

说明书

分子靶点：CD127, IL7R, IL7RA
种属：小鼠 (Mouse)
标记：PE
亚型：Rat IgG2a, κ
克隆号：A7R34

在售SKU, 70-AM012704-20, 70-AM012704-50, 70-AM012704-100, AM012704, 70-F2112702-25, 70-F2112702-100

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL7R 分子靶点信息概述
IL7R 分子靶点综述
- 小鼠 Mouse Il7r 分子靶点信息
- 小鼠 Mouse Il7r 靶点分子功能（预测）

商品名	Anti-Mouse CD127 (IL-7Rα), PE (Clone:A7R34) 流式抗体
靶点	CD127, IL7R, IL7RA
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Rat IgG2a, κ
克隆号	A7R34
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

分子信息

概述小鼠 CD127, IL7R, IL7RA 靶点信息

IL7R 分子靶点信息概述

分子名：IL7R, interleukin 7 receptor
基因家族：CD molecules; Fibronectin type III domain containing; Interleukin receptors
别名：CD127; IL7RA

IL7R 分子靶点综述

CD127是白细胞介素7受体α链，与CD132 (β链)共同形成高亲和力的受体。CD127表达于大部分T细胞，但在T细胞活化后可发生降低，也表达在B细胞前体和单核细胞。CD127的表达对T淋巴细胞的成熟、维持及免疫应答发挥着重要作用。

小鼠 Mouse Il7r 分子靶点信息

分子名：Il7r, interleukin 7 receptor
别称：
- CD127
- IL-7 receptor alpha chain
- IL-7Ralpha
基因序列：NCBI_Gene: 16197
蛋白序列：

小鼠 Mouse Il7r 靶点分子功能（预测）

Predicted to enable cytokine receptor activity. Involved in defense response to Gram-positive bacterium. Acts upstream of or within several processes, including hematopoietic or lymphoid organ development; negative regulation of T cell mediated cytotoxicity; and positive regulation of T cell differentiation in thymus. Located in external side of plasma membrane. Is expressed in adrenal gland; brain; early conceptus; hemolymphoid system; and lymphatic system. Human ortholog(s) of this gene implicated in severe combined immunodeficiency and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human IL7R (interleukin 7 receptor).

操作步骤

文章目录[隐藏]

分子信息
IL7R 分子靶点信息概述
IL7R 分子靶点综述
- 小鼠 Mouse Il7r 分子靶点信息
- 小鼠 Mouse Il7r 靶点分子功能（预测）
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 50 - 100 μl 抗凝血，加入 5 μl Anti-Mouse CD127 (IL-7Rα), PE 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Mouse CD127 (IL-7Rα), PE 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。300 × g 离心 5 - 10每管加入 1 - 2 ml 流式染色缓冲液，分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。