F21LY6G01

Anti-Mouse Ly-6G (Gr-1), FITC (Clone:RB6-8C5)流式抗体 (新品)

¥208.00 – ¥440.00

说明书

种属：小鼠 (Mouse)
标记：FITC
亚型：Rat IgG2b, κ
克隆号：RB6-8C5

在售SKU, 70-AM0L601-20, 70-AM0L601-50, 70-AM0L601-100, AM0L601, 70-F21LY6G01-25, 70-F21LY6G01-100

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Size
库存号

描述

商品名	Anti-Mouse Ly-6G (Gr-1), FITC (Clone:RB6-8C5)流式抗体
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Rat IgG2b, κ
克隆号	RB6-8C5
推荐应用	流式检测
用量	5 μL (0.5μg)/Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.09% NaN3 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 50 - 100 μl 抗凝血，加入 5 μl Anti-Mouse Ly-6G (Gr-1), FITC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Mouse Ly-6G (Gr-1), FITC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。300 × g 离心 5 - 10每管加入 1 - 2 ml 流式染色缓冲液，分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。