EK163HS

Human GM-CSF High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：CSF2, GM-CSF
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.07 pg/mL
检测范围：0.16 pg/mL - 10 pg/mL
回收率：80% - 101%

在售SKU:70-EK163HS-48, 70-EK163HS-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
CSF2 分子靶点信息概述
CSF2 分子靶点综述
- 人 Human CSF2 分子靶点信息
- 人 Human CSF2靶点分子功能（预测）

商品名	Human GM-CSF High Sensitivity ELISA Kit (人粒细胞-巨噬细胞集落刺激因子 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.6% - 5.5%；板间变异系数：4.8% - 5.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.07 pg/mL
检测范围	0.16 pg/mL - 10 pg/mL
回收率	80% - 101%
平均回收率	0.87
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗GM-CSF单克隆抗体的96孔聚苯乙烯酶标板人GM-CSF冻干标准品 GM-CSF检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人GM-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的GM-CSF与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中GM-CSF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human GM-CSF High Sensitivity ELISA Kit (人粒细胞-巨噬细胞集落刺激因子 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 CSF2, GM-CSF 靶点信息

CSF2 分子靶点信息概述

分子名：CSF2, colony stimulating factor 2
别名：GM-CSF; GMCSF
全称：sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)

CSF2 分子靶点综述

粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种调节白细胞生长的细胞因子。GM-CSF刺激干细胞产生粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)及单核细胞。此外，它对造血和非造血谱系细胞也具有活性。GM-CSF用作药物来刺激白细胞的产生，从而预防化疗后引起的中性粒细胞减少症。近来，人们正在临床试验评估GM-CSF作为一种潜在的疫苗佐剂对HIV感染患者的疗效。相关研究报道一些癌症(包括肺癌和急性髓细胞白血病)患者的血清中具有较高水平的GM-CSF，同样在支气管哮喘患者外周血和鳞屑提取物中也具有较高水平的GM-CSF。

人 Human CSF2 分子靶点信息

分子名：CSF2, colony stimulating factor 2
别称：
- colony stimulating factor 2 (granulocyte-macrophage)
- colony-stimulating factor
- CSF
- GMCSF
- granulocyte macrophage-colony stimulating factor
- granulocyte-macrophage colony stimulating factor
- granulocyte-macrophage colony-stimulating factor
- MGC131935
- MGC138897
- molgramostim
- molgramostin
- sargramostim
基因序列：NCBI_Gene: 1437
蛋白序列：UniProtKB: P04141

人 Human CSF2靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.