EK1244

Human HSP27/HSPB1 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：HSPB1, HSP27
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：5.86 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：92% - 114%

在售SKU:70-EK1244-48, 70-EK1244-96

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文章目录[隐藏]

分子信息
HSPB1 分子靶点信息概述
HSPB1 分子靶点综述
- 人 Human HSPB1 分子靶点信息
- 人 Human HSPB1靶点分子功能（预测）

商品名	Human HSP27/HSPB1 ELISA Kit (人热休克蛋白 (HSP) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.3% - 6.9%；板间变异系数：3.2%-4.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	5.86 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	92% - 114%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗HSP27/HSPB1单克隆抗体的96孔聚苯乙烯酶标板人HSP27/HSPB1冻干标准品人HSP27/HSPB1检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人HSP27预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的HSP27与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中HSP27的浓度成正比。加入终止液终止反应，在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。

Human HSP27/HSPB1 ELISA Kit (人热休克蛋白 (HSP) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 HSPB1, HSP27 靶点信息

HSPB1 分子靶点信息概述

分子名：HSPB1, heat shock protein family B (small) member 1
基因家族：Small heat shock proteins
别名：HSP27; HSP28; Hs.76067; Hsp25; CMT2F
全称：heat shock 27kD protein 1; heat shock 27kDa protein 1

HSPB1 分子靶点综述

热休克蛋白(HSP)是一组高度保守的应激蛋白，主要功能是作为分子伴侣，并参与多种生物学过程包括耐热性、抑制细胞凋亡、调节细胞发育和细胞分化、以及信号转导。 HSP27，又名HSPB1，是小分子热休克蛋白组中的分子伴侣。它存在于许多细胞类型中，尤其是所有类型的肌细胞，在细胞分化和发育的不同阶段过度表达。HSP27参与细胞凋亡信号途径，抑制procaspase-9的活化。此外，HSP27可保护肌动蛋白丝免受破碎，还可激活蛋白酶。HSP27可能是一个潜在的诊断标志物，高水平的HSP27存在于乳腺癌患者的血清中。磷酸化的HSP27增加人类前列腺癌细胞的浸润、促进细胞增殖并抑制Fas诱导的细胞凋亡。

人 Human HSPB1 分子靶点信息

分子名：HSPB1, heat shock protein family B (small) member 1
别称：
- 28 kDa heat shock protein
- CMT2F
- DKFZp586P1322
- epididymis secretory protein Li 102
- estrogen-regulated 24 kDa protein
- heat shock 27 kDa protein
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
- heat shock protein beta-1
- HEL-S-102
- HMN2B
- HS.76067
- HSP 27
- Hsp25
- HSP27
- HSP28
- putative heat shock protein family B member 1 isoform
- SRP27
- stress-responsive protein 27
基因序列：NCBI_Gene: 3315
蛋白序列：UniProtKB: P04792

人 Human HSPB1靶点分子功能（预测）

Enables several functions, including protein folding chaperone; protein homodimerization activity; and protein kinase binding activity. Involved in several processes, including anterograde axonal protein transport; positive regulation of angiogenesis; and positive regulation of endothelial cell migration. Located in cytoplasm and nucleus. Implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Biomarker of focal segmental glomerulosclerosis and rheumatoid arthritis.