EK174

Human IgA ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：待定
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
灵敏度：0.35 ng/mL
检测范围：3.13 ng/mL - 200 ng/mL
回收率：90% - 108%

在售SKU:70-EK174-48, 70-EK174-96

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Size
库存号

描述

商品名	Human IgA ELISA Kit (人免疫球蛋白AELISA 试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.4% - 4.8%；板间变异系数：0.7% - 6.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：10 μL；细胞培养上清：100 μL
灵敏度	0.35 ng/mL
检测范围	3.13 ng/mL - 200 ng/mL
回收率	90% - 108%
平均回收率	0.97
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IgA单克隆抗体的96孔聚苯乙烯酶标板人IgA冻干标准品 IgA 检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IgA抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IgA与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgA的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。