EK102HS

Human IL-2 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL2, IL-2
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.31 pg/mL
检测范围：3.91 pg/mL - 250 pg/mL
回收率：101% - 129%

在售SKU:70-EK102HS-48, 70-EK102HS-96, 人白介素2, 白介素2, 人, 白介素, 2

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL2 分子靶点信息概述
IL2 分子靶点综述
- 人 Human IL2 分子靶点信息
- 人 Human IL2靶点分子功能（预测）

商品名	Human IL-2 High Sensitivity ELISA Kit (人白介素2高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.2% - 4.9%；板间变异系数：6.0% - 11.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.31 pg/mL
检测范围	3.91 pg/mL - 250 pg/mL
回收率	101% - 129%
平均回收率	1.22
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-2单克隆抗体的96孔聚苯乙烯酶标板人IL-2冻干标准品 IL-2检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-2 High Sensitivity ELISA Kit (人白介素2高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

分子名：IL2, interleukin 2
基因家族：Interleukins
别名：IL-2; TCGF
全称：T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白，在人类中，由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子，调控对具有免疫功能的白血球(白细胞，通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答，区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能，其受体由3条链组成，分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角，包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

人 Human IL2 分子靶点信息

分子名：IL2, interleukin 2
别称：
- aldesleukin
- IL-2
- interleukin-2
- involved in regulation of T-cell clonal expansion
- lymphokine
- T cell growth factor
- TCGF
基因序列：NCBI_Gene: 3558
蛋白序列：UniProtKB: P60568

人 Human IL2靶点分子功能（预测）

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).