EK1133

Human myeloperoxidase/MPO ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：MPO, Myeloperoxidase
种属：人 (Human)
样本类型：血清，血浆，细胞上清
检测样本体积：100 μL
灵敏度：28.72 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：80% - 122%

在售SKU:70-EK1133-48, 70-EK1133-96

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库存号

描述

文章目录[隐藏]

分子信息
MPO 分子靶点信息概述
MPO 分子靶点综述
- 人 Human MPO 分子靶点信息
- 人 Human MPO靶点分子功能（预测）

商品名	Human Myeloperoxidase/MPO ELISA Kit (人髓过氧化物酶 (MPO) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.4% - 5.4%；板间变异系数：2.9% - 5.4%
样本类型	血清，血浆，细胞上清
检测样本体积	100 μL
灵敏度	28.72 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	80% - 122%
平均回收率	1.04
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Myeloperoxidase/MPO单克隆抗体的96孔聚苯乙烯酶标板人Myeloperoxidase/MPO冻干标准品 Myeloperoxidase/MPO检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MPO抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MPO与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MPO的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Myeloperoxidase/MPO ELISA Kit (人髓过氧化物酶 (MPO) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MPO, Myeloperoxidase 靶点信息

MPO 分子靶点信息概述

分子名：MPO, myeloperoxidase

MPO 分子靶点综述

髓过氧化物酶(MPO)是一种过氧化物酶，在人类中由位于17号染色体上的MPO基因编码。它是一种存在于中性粒细胞的嗜苯胺蓝颗粒中的溶酶体蛋白，在脱颗粒过程中释放到细胞外空间。MPO在中性粒细胞中表达最丰富，可产生具有抗菌活性的次卤酸。MPO有血红素色素，导致中性粒细胞中的分泌物呈现绿色，如脓。另外，MPO介导蛋白质亚硝基化、3-氯酪氨酸形成及二酪氨酸交联。MPO缺乏症是一种导致机体免疫缺陷的遗传性疾病。MPO水平的升高会大大加倍增加心血管死亡的风险。也有人认为，MPO在动脉粥样硬化病变发展中、致使斑块不稳定中起着重要作用。

人 Human MPO 分子靶点信息

分子名：MPO, myeloperoxidase
别称：
- 无
基因序列：NCBI_Gene: 4353
蛋白序列：UniProtKB: P05164

人 Human MPO靶点分子功能（预测）

Enables heparin binding activity and peroxidase activity. Involved in low-density lipoprotein particle remodeling. Acts upstream of or within hydrogen peroxide catabolic process. Located in azurophil granule; extracellular space; and nucleoplasm. Implicated in several diseases, including Alzheimer's disease 1; cystic fibrosis; kidney failure (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of asthma (multiple); glomerulonephritis; and lung disease (multiple).