EK1403

Human Total MMP-1 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,200.00

分子靶点：MMP1, CLG
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
灵敏度：1.81 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：84% - 117%

在售SKU:70-EK1403-48, 70-EK1403-96,EK1403

¥<\/span>2,000.00<\/bdi><\/span><\/span>","sku":"70-EK1403-48","variation_description":"","variation_id":20786,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3200,"display_regular_price":3200,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,200.00<\/bdi><\/span><\/span>","sku":"70-EK1403-96","variation_description":"","variation_id":20787,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

分子信息
MMP1 分子靶点信息概述
MMP1 分子靶点综述
- 人 Human MMP1 分子靶点信息
- 人 Human MMP1靶点分子功能（预测）

商品名	Human Total MMP-1 ELISA Kit (人总金属蛋白酶-1 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.1% - 6.4%；板间变异系数：2.6% - 5.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：10 μL；细胞培养上清：100 μL
灵敏度	1.81 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	84% - 117%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	酶标板标准品检测抗体标准品稀释液链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物(TMB)，避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human Total MMP-1 ELISA Kit (人总金属蛋白酶-1 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MMP1, CLG 靶点信息

MMP1 分子靶点信息概述

分子名：MMP1, matrix metallopeptidase 1
基因家族：Receptor ligands; M10 matrix metallopeptidases
曾用名：CLG
全称：interstitial collagenase; matrix metalloproteinase 1 (interstitial collagenase)

MMP1 分子靶点综述

基质金属蛋白酶1(MMP-1)，又名间质胶原酶和成纤维细胞胶原酶，在人类中由MMP1基因编码。MMP-1由成纤维细胞、软骨细胞、巨噬细胞、角质形成细胞、内皮细胞和成骨细胞产生。其产生受多种刺激物包括细胞因子(如EGF、白细胞介素和TNF-α)和化学物上调，受TIMP-1、TIMP-2和α2-巨球蛋白抑制。MMP-1在细胞外基质重塑中的胶原纤维降解中起重要作用，并参与多种生物过程包括类风湿性关节炎、骨关节炎、牙周病、肿瘤侵袭、血管形成、角膜溃疡、组织重塑、炎症性肠病、动脉粥样硬化和动脉瘤。另外，MMP-1同样也裂解多种底物如酪蛋白、明胶、蛋白聚糖、内功素、促肿瘤坏死因子和软骨连接蛋白。

人 Human MMP1 分子靶点信息

分子名：MMP1, matrix metallopeptidase 1
别称：
- CLG
- CLGN
- fibroblast collagenase
- interstitial collagenase
- matrix metallopeptidase 1 (interstitial collagenase)
- matrix metalloprotease 1
- matrix metalloproteinase 1
- matrix metalloproteinase 1 (interstitial collagenase)
基因序列：NCBI_Gene: 4312
蛋白序列：UniProtKB: P03956

人 Human MMP1靶点分子功能（预测）

Enables metalloendopeptidase activity. Involved in cellular response to UV-A; positive regulation of protein-containing complex assembly; and proteolysis. Predicted to be located in extracellular region. Implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including artery disease (multiple); arthritis (multiple); idiopathic pulmonary fibrosis; immunoglobulin light chain amyloidosis; and systemic lupus erythematosus.