EK373

Rat IgG2a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IgG
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞上清
检测样本体积：20 μL
灵敏度：22.52 pg/mL
检测范围：0.78 pg/mL - 50 pg/mL
回收率：80% - 110%

在售SKU:70-EK373-48, 70-EK373-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G, 免疫球蛋白2, 免疫球蛋白2a, 球蛋白2, 球蛋白2a, 免疫球蛋白G2, 免疫球蛋白G2a, 球蛋白G2, 球蛋白G2a, IgG2a, IgG2

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Size
库存号

描述

商品名	Rat IgG2a ELISA Kit (大鼠免疫球蛋白M ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.5% - 5.0%；板间变异系数：3.8% - 4.3%
样本类型	血清，血浆，细胞上清
检测样本体积	20 μL
灵敏度	22.52 pg/mL
检测范围	0.78 pg/mL - 50 pg/mL
回收率	80% - 110%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IgG2a单克隆抗体的96孔聚苯乙烯酶标板大鼠IgG2a冻干标准品 IgG2a检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IgG2a抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IgG2a与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG2a的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。