EK310

Rat IL-10 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IL10, CSIF, TGIF
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.48 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：86% - 108%

在售SKU:70-EK310/2-48, 70-EK310/2-96

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库存号

描述

文章目录[隐藏]

分子信息
IL10 分子靶点信息概述
IL10 分子靶点综述
- 大鼠 Rat Il10 分子靶点信息
- 大鼠 Rat Il10 靶点分子功能（预测）

商品名	Rat IL-10 ELISA Kit (大鼠白细胞介素10 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.4% - 5.3%；板间变异系数：4.5% - 6.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.48 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	86% - 108%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-10冻干标准品 IL-10检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-10 ELISA Kit (大鼠白细胞介素10 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

分子名：IL10, interleukin 10
基因家族：Interleukins
别名：CSIF; TGIF; IL10A; IL-10
全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

大鼠 Rat Il10 分子靶点信息

分子名：Il10, interleukin 10
别称：
- CSIF
- cytokine synthesis inhibitory factor
- If2a
- IL-10
- IL10X
- interferon 2a
- interleukin-10
基因序列：NCBI_Gene: 25325
蛋白序列：
- UniprotKB: A0A7R8C3K4
- UniprotKB: P29456

大鼠 Rat Il10 靶点分子功能（预测）

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).