EK171

Human IgG ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IgG
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：0.18 ng/mL
检测范围：3.13 ng/mL - 200 ng/mL
回收率：93% - 100%

在售SKU:70-EK171-48, 70-EK171-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G

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Size
库存号

描述

商品名	Human IgG ELISA Kit (人免疫球蛋白G ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：5.5% - 7.6%；板间变异系数：5.6% - 8.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	0.18 ng/mL
检测范围	3.13 ng/mL - 200 ng/mL
回收率	93% - 100%
平均回收率	1.04
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗人IgG单克隆抗体的96孔聚苯乙烯酶标板人IgG 冻干标准品 IgG 检测抗体辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IgG抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本进行孵育。经过孵育，样本中存在的IgG与固相抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。