EK273

Mouse IgG2a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IgG
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.015 ng/mL
检测范围：1.95 ng/mL - 125 ng/mL
回收率：90% - 110%

在售SKU:70-EK273-48, 70-EK273-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G, 免疫球蛋白2, 免疫球蛋白2a, 球蛋白2, 球蛋白2a, 免疫球蛋白G2, 免疫球蛋白G2a, 球蛋白G2, 球蛋白G2a, IgG2a, IgG2

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Size
库存号

描述

商品名	Mouse IgG2a ELISA Kit (小鼠IgG2a ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.5% - 7.5%；板间变异系数：4.6% - 5.4%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.015 ng/mL
检测范围	1.95 ng/mL - 125 ng/mL
回收率	90% - 110%
平均回收率	1.01
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IgG2a单克隆抗体的96孔聚苯乙烯酶标板小鼠IgG2a冻干标准品 IgG2a检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IgG2a抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本进行孵育。经过孵育，样本中存在的IgG2a与固相抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG2a的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。