EK8103

Prostaglandin E2/PGE2 Competitive ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,500.00 – ¥4,000.00

分子靶点：待定
种属：人 (Human); 小鼠 (Mouse); 大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：1.08 pg/mL
检测范围：15.63 pg/mL - 2000 pg/mL
回收率：92 - 102%

在售SKU:70-EK8103/2-48, 70-EK8103/2-96

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Size
库存号

描述

商品名	Prostaglandin E2/PGE2 Competitive ELISA Kit (前列腺素E2 竞争法ELISA试剂盒)
检测方法	竞争法
精密度	板内变异系数：3.9% - 4.3%；板间变异系数：5.2% - 6.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	1.08 pg/mL
检测范围	15.63 pg/mL - 2000 pg/mL
回收率	92 - 102%
平均回收率	0.99
板式	96孔板，可拆
保存	试剂盒未拆开，-20℃保存。已拆开，标准品、抗体、偶联物和酶-20℃保存，其它4℃保存。
运输条件	干冰运输
组分	包被抗小鼠IgG的96孔聚苯乙烯酶标板 PGE2冻干标准品 PGE2-biotin偶联物冻干粉 PGE2抗体冻干粉辣根过氧化物酶标记的链霉亲和素冻干粉检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。PGE2特异性的单克隆抗体加入至孔中，经过孵育，与固相抗体结合。洗涤后，加入生物素标记的PGE2和未标记的PGE2或样本，竞争结合单克隆抗体上有限的结合位点。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物，颜色反应的深浅与PGE2的浓度成反比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本，但其它哺乳动物的PGE2预计也可被检测。