EK376

Rat IgM ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：待定
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.17 ng/mL
检测范围：0.63 ng/mL - 40 ng/mL
回收率：80% - 110%

在售SKU:70-EK376-48, 70-EK376-96

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Size
库存号

描述

商品名	Rat IgM ELISA Kit (大鼠免疫球蛋白M (IgM) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.8% - 7.7%；板间变异系数：6.1% - 7.1%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.17 ng/mL
检测范围	0.63 ng/mL - 40 ng/mL
回收率	80% - 110%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IgM单克隆抗体的96孔聚苯乙烯酶标板大鼠IgM冻干标准品 IgM检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠 IgM 单克隆抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和检测样本进行孵育。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 IgM 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值