F1100301

Anti-Human CD3, FITC (Clone:OKT3)检测试剂

¥240.00 – ¥528.00

分子靶点：CD3
种属：人 (Human)
标记：FITC
亚型：Mouse IgG2a, κ
克隆号：OKT3

在售SKU, 70-AH00301-20, 70-AH00301-50, 70-AH00301-100, AH00301, 70-F1100301-25, 70-F1100301-100, 人, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3, CD3

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Size
库存号

描述

文章目录[隐藏]

结果示例

商品名	Anti-Human CD3, FITC (Clone:OKT3)检测试剂
靶点	CD3
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Mouse IgG2a, κ
克隆号	OKT3
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD3, FITC (F1100301)和 Anti-Human CD19, APC (F1101903)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

操作步骤

文章目录[隐藏]

结果示例
使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD3, FITC 和其他抗体，震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 ×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD3, FITC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。